The second game in the Zero Escape series. Zero Escape: Virtue's Last Reward is a mystery/thriller visual novel. It is story focused with multiple endings that use player choices and puzzle rooms to progress. Additions to the game include an 'auto-advance text' mode, so players can listen to the voice acting without clicking through dialog, a memo function that allows players to note anything they feel is useful, and a flow chart that shows how alternate choices unfold. A calculadora do jogo foi removida.

"Ele acordou em um elevador. Across the room was a strange girl he’d never met before, even though she seemed to know him... “Sigma...” “How do you know my name?” he asked, but before she could answer a rabbit appeared. “This elevator is about to fall!” Sigma e a garota saíram do elevador apenas para se encontrarem em uma sala enorme com outros sete estranhos...nove pessoas ao todo. The strange rabbit told them that they would have to play the Ambidex Game-a game of betrayal. A penalidade por perder era a morte. Could these nine strangers trust one another long enough to escape their prison alive?"